Fig. 5

Screenshots of the main image analysis steps performed with CecogAnalyzer. a Object detection—primary channel. Object detection processing step for primary channel corresponding to Hoechst 33342 fluorescence. The contours in red correspond to the nuclear region of the cells, and define as such the number of cells per each time frame. b Object detection—secondary channel. Object detection processing step for secondary channel corresponding to pHrodo™Red fluorescence. The contours in green correspond to the area around the nucleus in which the pHrodo™Red signal (displayed in white) can be detected. c Manual annotation in the annotation browser. Examples picked for the two classes “nanonegative” and “nanopositive.” Hoechst 33342 fluorescence in blue, pHrodo™Red signal in red. “Nanopositive” cells indicated by “2”, “nanonegative” cells by “1.” d Automatic classification of cells approximately 3 h post-incubation with NWs. “Nanopositive” cells indicated by the yellow contour. “Nanonegative” cells indicated by the green contour