Express photolithographic DNA microarray synthesis with optimized chemistry and high-efficiency photolabile groups

Table 3 Representative chemical synthesis protocol, in Expedite 8909 format, used for the express syntheses

Cycle SPh-NPPOC-dT (express synthesis)
Function	Mode	Pulses	Sec	Description
$Coupling
1/*Wsh	Pulse	10	0	Flush with Wsh
2/*Act	Pulse	6	0	Act
21/*T + Act	Pulse	5	0	T + Act
2/*Act	Pulse	6	0	Push with Act
1/*Wsh	Pulse	3	15	Couple monomer
1/*Wsh	Pulse	10	0	Flush with Wsh
$Capping
40/*Gas A	Pulse	1	10	Dry column
$Oxidizing
15/*Ox	Pulse	3	0	Ox to column
12/*Wsh A	Pulse	10	0	Flush with Wsh A
17/*Aux	Pulse	15	0	Exposure solvent
130/*Event 2	Na	4	1	Event 2 out
12/*Wsh A	Pulse	5	9	Push with Wsh A
130/*Event 2	Na	4	1	Event 2 out
12/*Wsh A	Pulse	10	0	Flush with Wsh A

The 9 s exposure, at 50 mW/cm², corresponds to an exposure of 0.45 J/cm². The five pulses of Wsh A during the exposure push the exposure solvent towards the waste, but the reaction chamber remains full of exposure solvent until the next washing step. The short oxidation step in this synthesis, the first two steps after the $Oxidizing header, are used after every coupling

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