Fig. 2

Fluorescent and cryo-SXT correlative workflow. a In vivo differential interference contrast (DIC) image of MCF-7 cells cultured on Au-HZBII grid and incubated 24 h with SPION (0.25 mg ml⁻¹). Bar 200 μm. b In vivo fluorescent image from the area in the yellow square in a. Bar 20 μm. Nucleus, blue (DAPI), acidic vesicles, red (LysoTracker Red). c Cryo-epifluorescent image (red channel) from the area in the yellow square in b. Bar 5 μm. d Cryo-SXT plane from the area in the yellow square in c. N, nucleus. Bar 2 μm. e Cryo-SXT plane showing ultrastructural details of the cell. Arrowheads indicate mitochondrial cristae. Bar 500 nm. f Volumetric representation of the tomogram in d. High-absorption vesicles (red), segmented applying a threshold adapted to the volume containing the highest densities, are condensed near the nucleus (blue), displacing the mitochondrial network (yellow). Grey filaments, orange plasma membrane. Dataset acquired at HZB-BESSYII