Fig. 6

Focal adhesion formation/dynamics and cytoskeletal organisation differ on flat or neuritogenesis-inducing nanostructured surfaces. a The graphic visualizes and summarizes the cytoskeletal and mechanobiological processes which are influenced by FA organization and dynamics. b In the panel representative images of the PC12 cells fixed with 4 % PFA after the indicated time periods on the different surfaces are demonstrated (vinculin staining recorded by TIRF microscopy, f-actin in epifluorescence). The white arrows indicate exemplary areas with focal complex (pointed line) or focal adhesion (continuous line) structures. The asterisks pinpoint to exemplary areas with strong actin fiber formation. c–e The graphs summarize the corresponding results (representing the global statistics of three independent experiments, vinculin clusters: n = 722–3678, cells: n = 16–34) of the quantifications (obtained with ImageJ) of c, d FA dynamics; c Vinculin cluster area, d Number of vinculin clusters per cell, and e cytoskeletal actin fiber organization. na not analyzable