Fig. 8

The interaction with the neuritogenesis-inducing surface has an impact on transcription factor dynamics relevant for neuronal differentiation. a The confocal images show the average stack projection of p-CREB stainings of cells in the indicated experimental conditions. The cells were fixed with 4 % PFA 60 min or 24 h after in the indicated conditions and stained for the nucleus (HOECHST), f-actin and p-CREB. The outer dashed lines represent the outlines of the cells obtained from the f-actin staining and the inner dashed line the nuclear area determined from the HOECHST staining. The graph (representing the global statistics of two independent experiments, n = 33–76 cells) summarizes the corresponding quantification performed with the help of ImageJ (see also “Methods”). b The same experimental procedure and quantification (from three independent experiments, n: > 500 cells, >150 neurites) as in Additional file 2: Figure S2A–C, but with an inhibitor against JNK/c-jun (SP600125 10 and 20 µM). Representative images of the conditions can be found in Additional file 3: Figure S3