Fig. 3

Representative photographs of fluorescent images of SR-FLIVO (red, b, f) and TUNEL staining (green, c, g) on the non-ischemic side (a–d) and the ischemic side (e–h). The ROI is set in yellow squares in the non-ischemic area (a) and the ischemic area (e). Merged images of SR-FLIVO and TUNEL staining are shown in d and h. Almost no positive fluorescent expression was observed on the non-ischemic side (a–d). SR-FLIVO expression (f) and TUNEL immunopositivity (g) were observed in the region around the ischemic penumbra. Intracellular co-localization of these two immunopositivities can be detected in the merged image (orange, h). Bars 100 μm