Fig. 6

FRA modulates HuR-FNP efficacy. a HuR-FNP-mediated growth inhibition of H1299 cells was diminished in the presence of excess of folic acid (1 mM) in the culture medium compared to HuR-FNP inhibition in the absence of excess folic acid. HuR-FNP-mediated inhibitory activity on the expression of b HuR protein and c mRNA expression levels were also abrogated the presence of excess of folic acid (1 mM) compared to HuR-FNP treatment in the absence of excess folic acid in H1299 cells. d HuR-FNP-mediated growth inhibition was less in CCD16 cells that was further diminished in the presence of excess of folic acid (1 mM). e HuR protein and f mRNA expression levels were not markedly affected in CCD16 cells in the presence of excess folic acid compared to in the absence of excess folic acid. (error bars denote SD; *p < 0.05; **p < 0.01)