Fig. 7From: Designing, construction and characterization of genetically encoded FRET-based nanosensor for real time monitoring of lysine flux in living cellsIn vivo analysis of the FLIPK (WT) nanosensor. a FRET ratio change in response to the 10 mM lysine (black circles) and without lysine (grey circles) in 20 mM PBS buffer. b FRET (535/485 nm) ratio of the bacterial cell suspension before and after incubation with various amino acids for 45 minBack to article page