Fig. 1

Light micrographs of CA1 hippocampal subfield of rats 1 h after i.v. injection of vehicle (a) and 15 min after i.v. injection of rGO (b), Hematoxylin-eosin. Insets show greater magnification of hippocampal pyramidal neurons with preserved euchromatic nuclei and visible nucleoli both in control and treated samples. Or stratum oriens; Py stratum pyramidale; Rad stratum radiatum. NeuN and GFAP labeling of 1 h-control rats (c, e) and 15 min after i.v. injection of rGO (d, f), respectively. Western blot signals of NeuN (g) and GFAP (h) after administration of rGO (7 mg/kg) in hippocampal tissue lysates were quantified densitometrically and normalized to an internal standard protein (β-actin). The results were shown as a percentage of control (100 %), and represent mean ± SEM (n = 5 rats/interval). *p < 0.05 indicates significant difference relative to control. One-way ANOVA, Bonferroni post hoc test; Bars 100 µm (a–f), 50 µm (insets)