Fig. 4

Lipo-EF24 inhibits proliferation and induces apoptosis in pancreatic cancer cells. a CFSE staining assays were used to measure the effect of Lipo-EF24 on pancreatic cancer cell proliferation. CFSE-labeled MIAPaCa and Pa03C cells were treated with Lipo-EF24 at concentrations of 5 and 10 µM or void liposomes for 48 h and then analysed using flow cytometry. Proliferation was indicated by a decrease of fluorescence intensity. b In order to determine apoptosis rates, MIAPaCa and Pa03C cells were treated with indicated concentrations of void liposomes or Lipo-EF24 for 24 h and protein expression levels of cleaved PARP or procaspase-3 were quantified from whole cell lysates by immunoblotting