Fig. 6

LPFFD and KLVFF vectorized nanoparticles hybridization on brain slices of APPswe/PS1A246/TTR transgenic (a, c, e, f, g) and control (b, d, h) mice. Imaging beta-amyloid plaques were visualized owing to Cy5.5 fluorescence grafted to AGuIX@PEG@LPFFD@Cy5.5 (zoom in a), and AGuIX@PEG@KLVFF@Cy5.5 (zoom in c), in the near-infra red (yellow color, λexc = 620 nm-λem = 642 nm). Cellular nucleus of brain sections were located using Hoechst dye staining (blue color λexc = 350 nm-λem = 405 nm). AGuIX@PEG@LPFFD@Cy5.5 and AGuIX@PEG@KLVFF@Cy5.5 binding specificity test were performed on brain section without amyloid plaques (b, d), and with nanoparticle without peptides, AGuIX@PEG@Cy5.5 (e), or with a non-related Aβ(1-42) peptide, AGuIX@PEG@TTR-peptide@Cy5.5 (f) on brain section with amyloid plaques. Positive control of amyloid burden was performed using PIB staining on brain sections with (g) and without amyloid plaques (h). Ligands binding are detected by fluorescence imaging of PIB at 400 nm (blue color, g). Tissue architecture was highlighted by imaging actin-phalloidin staining (g, h, red color, λexc = 540 nm-λem = 570 nm)