Fig. 7
a, b MSC proliferation curves. a Cells were incubated for 24 h with DMEM-LG (filled circle), or with DMEM-LG with diluted γ-Fe2O3-DMSA (filled square) (80 μg/mL), then were plated and counted after different times. There was no significant difference between the experimental groups (p > 0.05) in any count times. b Cells were incubated for 24 h with DMEM-LG (filled circle), or with DMEM-LG with diluted Au-DMSA (filled square) (90 μg/mL), then were plated and counted after different times. (*) Significant increase in Au-DMSA labeled MSC amount compared to the control group only on the second day (p < 0.05). c Analysis by flow cytometry of CFSE-marked lymphocytes, co-cultured with labeled and unlabeled MSC. The spectra shown are representative of assays performed in triplicate. Gray Line Lymphocytes not marked with CFSE; Black line not activated marked lymphocytes; Red Line activated marked lymphocytes; Blue line activated lymphocytes co-cultured with MSC; Yellow line activated lymphocytes co-cultured with γ-Fe2O3-DMSA labeled MSC; Green line activated lymphocytes co-cultured with Au-DMSA labeled MSC