Fig. 4
From: Wnt3a nanodisks promote ex vivo expansion of hematopoietic stem and progenitor cells
Gel filtration chromatography of Wnt3a ND. A Wnt3a ND preparation was subjected to size exclusion chromatography with collection of 1 ml fractions (a). The elution position of standards (670, 132, 66 and 0.4 kDa) is depicted by arrows and the absorbance 280 profile for Wnt3a ND elution is shown in green. The data are representative of two independent experiments. A portion of selected fractions was analyzed by immunoblot (b) by probing with anti-Wnt3a (see orange line in a) and anti-apoA-I (see blue line in a)