Fig. 7

Effect of Wnt3a formulations on LSK cell proliferation/self-renewal. One thousand LSK cells were seeded per well and cultured as described. After 6 days, three wells were combined, counted and analyzed by flow cytometry. Values reported are normalized to cells cultured in medium supplemented with buffer only (for empty ND) or buffer containing equivalent amount of CHAPS (for Wnt3a ND and Wnt3a CHAPS). a Total cell count by hemocytometry. Control wells contained 87,000 ± 6000 (buffer only) and 76,000 ± 6000 (buffer with CHAPS) cells per well. b The LSK cell count obtained by FACS analysis [final numbers for control were 4690 ± 780 (buffer only) and 3650 ± 520 (buffer with CHAPS)]. p values (versus control) are shown above each column. The data present averages of four independent experiments