Fig. 5

IONP-induced alterations in cell area (grey bars) and cell circularity (orange lines) visualized after labelling of the cytoplasm with the CellMask™ Blue probe for the NSC, progenitor cell lines and murine neuroblastoma cell line. Cell circularity is a measure of cell spreading and is a value between zero and one, where one represents a perfect sphere. LA-N-2 cell morphology was analysed in terms of cluster area (grey bars) and number of cells per cluster (orange bars). A decreased cell area and increased cell circularity were detected in the NSC, ReNcell and Neuro-2a cell line. For the C17.2 cells only a diminution in cell area was detected. In the LA-N-2 cell line a reduction in cells per cluster and cluster size were observed. Statistical significance is indicated when appropriate (*p < 0.05), in black for the cell area and orange in case of the cell circularity (respectively cluster area and cells per cluster in case of the LA-N-2 cell line). NTC not treated control