Fig. 3
An-NPs attenuated Aβ pathology and prevents Aβ1–42-induced activation of P38/JNK Pathways. a Effect of An-NPs treatment on the expression of Alzheimer markers analyzed by Western Blot in Aβ1–42 treated SHSY-5Y cell line. Shown are representative Western Blots probed with antibodies of APP and BACE-1 in the Aβ1–42 treated SHSY-5Y cells. Data are the representative of three individual experiments (n = 3). The protein bands were quantified using sigma gel software. β-Actin was used to show equivalent amounts of protein loading. b Representative Western Blots of p-P38, p-JNK, p-NF-kB, TNF-α and iNOS in Aβ1–42-treated SH-SY5Y cells. Data are the representative of three individual experiments (n = 3). The protein bands were quantified using sigma gel software. β-Actin was used to show equivalent amounts of protein loading. c The double immunofluorescence images of SH-SY5Y cells after Aβ1–42 and An-NPs treatment, showing p-JNK (green) and BACE-1 (red), proteins and their respective relative density histograms. The DAPI (blue) was used to stain the nucleus. All the experiments were performed in triplicate. The details are given in the "Methods" section. *Significantly different from the control; #significantly different from Aβ1–42-treated group. Significance = **p < 0.01, #p < 0.05, ##p < 0.01