Fig. 4
An-NPs treatment alleviated oxidative stress and upregulated Nrf-2 and its downstream targets genes HO-1 expressions. a Shown are representative Western Blots probed with antibodies of Nrf2, and HO-1 in the Aβ1–42 treated SHSY-5Y cells. Data are the representative of three individual experiments (n = 3). The protein bands were quantified using sigma gel software. β-Actin was used to show equivalent amounts of protein loading. b The double immunofluorescence images of SH-SY5Y cells after Aβ1-42 and An-NPs treatment, showing 8-Oxo-G (green) and Nrf2 (red), proteins and their respective relative density histograms. The DAPI (blue) was used to stain the nucleus. All the experiments were performed in triplicate. The details are given in the “Methods” section. *Significantly different from the control; #significantly different from Aβ1–42-treated group. Significance = **p < 0.01, #p < 0.05, ##p < 0.01