Fig. 5
The beneficial effects of An-NPs against Aβ1–42-induced apoptosis and neurodegeneration; a effect of An-NPs treatment on the activation of apoptotic induced proteins analyzed by Western Blot in Aβ1–42 treated SH-SY5Y cell line. Induction of Bax, Bcl-2 and caspase-3 in Aβ1–42-treated SH-SY5Y cells were analyzed by Western Blot. Data are the representative of three individual experiments (n = 3). The protein bands were quantified using sigma gel software. β-Actin was used to show equivalent amounts of protein loading. b Representative photomicrographs of TUNEL and DAPI stained cells (magnification ×10 objective field, scale bar 50 µm) showing a high number of apoptotic neurons in Aβ1–42-treated group and comparatively low number of apoptotic neurons in Aβ plus An-NPs treated group. Data are the mean value (±SEM) for three independent experiments (n = 3). *Significantly different from the control; #significantly different from Aβ1–42-treated group. Significance = **p < 0.01, ***P < 0.001, #p < 0.05, ##p < 0.01, ###p < 0.001