Fig. 2
A Cellular near-infrared fluorescence and GPMQNs uptake. Inverted fluorescence microscopy of HepG2/ADM cells with 10 mg L−1 GPMQNs, B Control (Scale bar 50 μm). B 3D reconstruction of HepG2/ADM cells treated with 10 mg L−1. GPMQNs of near-infrared fluorescence for intracellular distribution (Scale bar 50 μm). D Whole body optical imaging examination of HepG2/ADM cells incubated with identical 10 mg L−1 GPMQNs solutions after 24 h incubation; a Control, b 1 mg L−1 red fluorescent modified miR-122, c 10 mg L−1 GPMQNs containing the red fluorescent modified miR-122. E Quantitative assay of GPMQNs on cell membrane permeability based on the LDH release assay; 1 untreated control, 2 resistant HepG2/ADM cells transfected with miR-122 (1 mg L−1, same concentration as loaded on GPMQN), 3 resistant HepG2/ADM cells incubation with 10 mg L−1 GPMQNs without miR-122, 4 resistant HepG2/ADM cells incubation with 10 mg L−1 GPMQNs (*P < 0.05 compared to the control group)