Fig. 5

Endocytic pathways involved in PLA-NP uptake. Representative images acquired as Z-stacks by confocal microscopy of three independent experiments. A549 cells were treated with inhibitors for different endocytic pathways followed by 60 min incubation with 20 µg/mL PLA-NP. On the left, the inhibited pathways are indicated, instead of the inhibitors used. Green dots refer to PLA-NP, red and blue staining to actin and nuclei, respectively. The mid-lane represents maximum projections of at least 25 optical sections of the Z-stacks. The images on the right lane are single-slices from the corresponding Z-stacks and the cross-hair set indicates evidence of the PLA-NP internalization (the cross-section of the sample in the yz-plane is shown on the right and in the xz-plane at the bottom). Scale bar 30 µm