Non-invasive continuous monitoring of pro-oxidant effects of engineered nanoparticles on aquatic microorganisms

Table 2 Selection of H₂O₂ detection methods [14]

Technique/probe	Observable	LOD	Application notes	Reference
Direct detection	Absorbance of H₂O₂	mM		[55, 56]
Cyt c	Absorbance	nM	Optimal reaction in low ionic strength solutions	[57, 58]
Xylenolorange + Fe³⁺	Absorbance of complex	μM	Carried out in acidic acids	[59]
Xylenolorange + Ti⁴⁺	Absorbance of complex	μM	Carried out in acidic acids	[59]
Luminol	Chemi-luminescence	nM	Interference with Mn²⁺ and Fe³⁺	[60–62]
2,7-Dichlorodihydrofluorescein (DCFH)	Fluorescence of product	pM-nM	Can be oxidised by other ROS	[16, 63]
p-Hydroxyphenylacetic acid	Fluorescence of product	nM	Optimal reaction at pH > 8.5	[64, 65]

ISSN: 1477-3155