Fig. 3

Dynamic interaction between QDs-RBVs and acidic endosomes. a Fluorescence images of QDs-RBVs, LysoTracker Green labeled acidic endosomes, and their merge (arrows) in Sf9 cells. b Typical time-lapse images of a QDs-RBV (red) entering into an acidic endosome (green). Velocity vs time plots (c) and MSD vs time plots (d) corresponding to b. The red curve and blue line in d is the fit to \({\text{MSD}} = 4D\tau + (V\tau )^{ 2} + {\text{constant}}\) and \({\text{MSD}} = 4D\tau^{\alpha } + {\text{constant}}\), respectively. Typical velocity vs time plots (e) and MSD vs time plots (f) of the QDs-RBVs in Sf9 cells with (blue) and without (red) Bafilomycin A1 treatment. The red curve and blue line in f is the fit to \({\text{MSD}} = 4D\tau + (V\tau )^{ 2} + {\text{constant}}\) and \({\text{MSD}} = 4D\tau^{\alpha } + {\text{constant}}\), respectively. g Histograms for the yields of baculoviruses propagated in Sf9 cells without or with Bafilomycin A1 treatment