Fig. 9
Productions of the HIDPPDNE-induced singlet oxygen under NIR laser irradiation. Upon exposure to an 808-nm laser with an intensity of 6 W/cm2, the productions of singlet oxygen generated from the (a) HIDPPDNEs with and without preoxygenated treatment and (b) freely dissolved ICG in PBS with concentrations of 0- (PBS only), 0.5-, 1-, 2-, 4-, and 10-μM ICG equivalent were measured every 60 s for 5 min. The quantities of singlet oxygen were analyzed based on the intensities of the SOSG-induced fluorescence measured by using spectrofluorometry with excitation/emission wavelength of 488/525 nm. Values are mean ± s.d. (n = 3)