Fig. 4

a FACS results showing the time-dependent increase in the ROS production after 2 µg Se/ml SeNP treatment for 6, 9, 12, 15, 18, 21 or 24 h. DHR 123 was used as a mitochondrial ROS indicator. A clear shift in the cell population with maximum ROS production was observed after 15 h of treatment. b Comparative analysis of the shift in the PC-3 cell population after 6, 12, 18 or 24 h of treatment with SeNPs. Experiments were performed in triplicate