Fig. 1

From top to bottom the schematic drawing of the constructs SAPN-P27-1, SAPN-P27-2, SAPN-P27A-1, SAPN-P27A-2 and SAPN-Combo are shown. Coiled coils are represented by thick boxes with the pentamer in green and the trimer in blue color. The non-coiled-coil structures are shown in thin boxes. The color coding is as follows: B-cell epitopes—red; CD4-epitopes—magenta; CD8-epitopes—purple; His-tag—black. The P27 B-cell epitope (red; N-terminal [P27-N] part solid; C-terminal [P27-C] part striped) is part of the trimeric coiled coil while the unstructured P27A (red with white dots) is attached at either the C-terminal or the N-terminal end of the coiled coil