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Table 2 Summarised results of the universal RNA standard spiked before transcription (part 1), which shows initial descriptive statistics and secondary correlations, as generated by BestKeeper analyses

From: The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

 

Least stable

Most stable

SD

P P I (0.61)

18S (0.59)

HPRT1 (0.53)

ACTB (0.42)

TBP (0.36)

HSP90 (0.27)

GAPDH (0.26)

YWHAZ (0.26)

SDH (0.26)

GUSB a (0.23)

CV

1 8 S (3.80)

PPI (3.15)

ACTB (2.36)

HPRT1 (2.33)

GAPDH (1.46)

TBP (1.44)

HSP90 (1.32)

YWHAZ (1.22)

SDH (1.09)

GUSB b (0.96)

r

HPRT1 (0.396)

TBP (0.499)

GAPDH (0.529)

ACTB (0.546)

PPI (0.630)

GUSB (0.681)

HSP90 (0.797)

YWHAZ (0.875)

SDH (0.903)

18S c (0.957)

  1. SD: standard deviation (a stability = ranked low to high)
  2. CV: coefficient of variance (b stability = ranked low to high)
  3. r: Pearson correlation coefficient (c stability = ranked high to low)