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Table 6 Summarised results of the universal RNA standard spiked at the amplification step (part 2), showing initial descriptive statistics and secondary correlations, as generated by BestKeeper analyses

From: The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

  Least stable Most stable
SD PPI (0.89) SDH (0.63) GAPDH (0.50) HPRT1 (0.46) HSP90 (0.42) TBP (0.40) YWAHZ (0.37) ACTB (0.36) 18S (0.36) GUSB a (0.35)
CV PPI (4.56) GAPDH (2.78) SDH (2.67) 18S (2.54) HSP90 (2.10) ACTB (2.02) HPRT1 (1.99) YWHAZ (1.74) TBP (1.58) GUSB b (1.46)
r YWHAZ (0.634) PPI (0.694) SDH (0.729) ACTB (0.821) GAPDH (0.845) HPRT1 (0.882) HSP90 (0.906) TBP (0.913) GUSB (0.980) 18S c (0.994)
  1. SD: standard deviation (a stability = ranked low to high)
  2. CV: coefficient of variance (b stability = ranked low to high)
  3. r: Pearson correlation coefficient (c stability = ranked high to low)