Fig. 1
From: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein
Construct for expression of trimeric Haemagglutinin in plants. H7 was expressed in tobacco leaves under the control of the CaMV 35S promoter. H7 was fused with trimeric motif GCN4-pII to form H7-pII. Recombinant H7 contained His and c-myc tags for affinity chromatography purification and Western blotting, respectively. The LeB4 signal peptide and KDEL motif were used to ensure ER retention. 35S-P: CaMV 35 S promoter; SP: legumin B4 signal peptide; H7: haemagglutinin A/H7N9; GCN4-pII: trimeric motif; His: His-tag; 35S-T: CaMV 35S terminator