Fig. 7

Viability of adipose-derived mesenchymal stem cells cultured on nanocomposites. a representative micrographs of cell viability assays at 24 h of culture; live cells (calcein positive cells) are marked in green and dead cells (EthD-1 positive cells) are marked in red. CTL (control) corresponds to cells cultured on culture multiwell plates. Graph b shows cell viability percentage for each experimental condition. Graph c shows the total number of cells (live and dead) present on CTL, RPS and RPS-AgNPs nanocomposites after 24 h of culture. Graph d shows the number of viable cells detached from CTL, RPS and RPS-AgNPs nanocomposites at different culture times (cell proliferation curves), for up to 5 days of culture. Graph e shows the amount of metabolically active cells (as indirectly evaluated from absorbance measurements by MTT assay) over time, after cells exposure to RPS or AgNPs-RPS supernatants or CTL (cells exposed to fresh DMEM complemented with 10% FBS and 1% penicillin/streptomycin): For all graphs, ***p < 0.0001vs CTL; ANOVA, Tukey post hoc test