Fig. 3
From: Near-infrared quantum dots labelled with a tumor selective tetrabranched peptide for in vivo imaging
NT4-QDs binding by flow cytometry. a Flow cytometry analysis showing binding of 20 nM NT4-QDs (light grey) and unlabelled QDs (dark grey) to HT29 cells. b Mean fluorescent intensity using different concentrations of NT4-QDs and unlabelled QDs. ***p < 0.001 calculated using one tailed Student t test. NT4-QDs (c) and unlabelled QDs (d) binding in the presence of NT4. NT4-QDs (e) and unlabelled QDs (f) binding in the presence of heparin. Flow cytometric analysis on 10,000 events was done using a BD FACSCanto II instrument (BD, NJ. USA) using a blue laser dye and the PerCP-Cy5-5-A channel