Fig. 4

Direct visualization of SR-B1-binding abilities of various lipoproteins. a, b AFM topographical images of a SR-B1 monolayer (a) and anti-SR-B1 antibody particles (b); c–i AFM topographical images of BSA (c), anti-SR-B1 antibody (d), and lipoproteins (e–i: HDL, LDL, oxLDL, acLDL, and VLDL, respectively) binding to a SR-B1 monolayer. Scan range: 1 μm × 1 μm. Right panels: the images are showed in multicolor to clearly distinguish the binding proteins/lipoproteins (green) from the SR-B1 monolayer (red). j Quantification of the average number of SR-B1-binding antibody/lipoprotein particles in a scan range. p < 0.05 for all pairs of columns except for LDL vs. VLDL