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Table 1 Values of the matrix of a factorial design of sonication parameters and measured responses

From: Memantine loaded PLGA PEGylated nanoparticles for Alzheimer’s disease: in vitro and in vivo characterization

  Amplitude 1st sonication time 2nd sonication time Zav (nm) PI ZP (mV) EE (%)
Coded level (%) Coded level (s) Coded level (s)
Factorial points
F1 − 1 25.0 − 1 20.0 − 1 120.0 390.4 ± 2.2 0.213 ± 0.039 − 6.73 ± 0.04 3.46
F2 1 35.0 − 1 20.0 − 1 120.0 249.7 ± 4.7 0.069 ± 0.022 − 6.33 ± 0.49 9.59
F3 − 1 25.0 1 40.0 − 1 120.0 184.6 ± 0.7 0.125 ± 0.023 − 6.43 ± 0.45 42.57
F4 1 35.0 1 40.0 − 1 120.0 227.0 ± 2.6 0.057 ± 0.019 − 6.72 ± 0.33 36.89
F5 − 1 25.0 − 1 20.0 1 240.0 243.0 ± 0.9 0.194 ± 0.012 − 6.72 ± 0.24 7.43
F6 1 35.0 − 1 20.0 1 240.0 248.1 ± 1.9 0.053 ± 0.037 − 6.48 ± 0.15 14.69
F7 − 1 25.0 1 40.0 1 240.0 258.7 ± 4.5 0.198 ± 0.011 − 6.35 ± 0.33 22.63
F8 1 35.0 1 40.0 1 240.0 206.4 ± 1.2 0.061 ± 0.045 − 6.67 ± 0.30 2.88
Axial points
F9 1.68 38.4 0 30.0 0 180.0 222.4 ± 2.4 0.033 ± 0.011 − 5.63 ± 0.37 39.12
F10 − 1.68 21.6 0 30.0 0 180.0 162.6 ± 0.4 0.262 ± 0.012 − 6.83 ± 0.37 39.36
F11 0 30.0 1.68 47.0 0 180.0 226.7 ± 4.4 0.236 ± 0.011 − 6.49 ± 0.25 19.94
F12 0 30.0 − 1.68 13.0 0 180.0 196.8 ± 2.5 0.103 ± 0.056 − 6.47 ± 0.55 43.10
F13 0 30.0 0 30.0 1.68 281.0 239.8 ± 0.7 0.056 ± 0.020 − 5.77 ± 0.47 23.39
F14 0 30.0 0 30.0 − 1.68 79.0 382.6 ± 5.2 0.221 ± 0.011 − 5.93 ± 0.21 33.95
Center points
F15 0 30.0 0 30.0 0 180.0 220.1 ± 5.6 0.059 ± 0.019 − 5.36 ± 0.03 24.01
F16 0 30.0 0 30.0 0 180.0 222.1 ± 3.6 0.062 ± 0.021 − 5.36 ± 0.11 23.23
  1. Italic values correspond to the optimized formulation of MEM loaded NPs