Table 1 Values of the matrix of a factorial design of sonication parameters and measured responses
Amplitude | 1st sonication time | 2nd sonication time | Zav (nm) | PI | ZP (mV) | EE (%) | ||||
|---|---|---|---|---|---|---|---|---|---|---|
Coded level | (%) | Coded level | (s) | Coded level | (s) | |||||
Factorial points | ||||||||||
F1 | − 1 | 25.0 | − 1 | 20.0 | − 1 | 120.0 | 390.4 ± 2.2 | 0.213 ± 0.039 | − 6.73 ± 0.04 | 3.46 |
F2 | 1 | 35.0 | − 1 | 20.0 | − 1 | 120.0 | 249.7 ± 4.7 | 0.069 ± 0.022 | − 6.33 ± 0.49 | 9.59 |
F3 | − 1 | 25.0 | 1 | 40.0 | − 1 | 120.0 | 184.6 ± 0.7 | 0.125 ± 0.023 | − 6.43 ± 0.45 | 42.57 |
F4 | 1 | 35.0 | 1 | 40.0 | − 1 | 120.0 | 227.0 ± 2.6 | 0.057 ± 0.019 | − 6.72 ± 0.33 | 36.89 |
F5 | − 1 | 25.0 | − 1 | 20.0 | 1 | 240.0 | 243.0 ± 0.9 | 0.194 ± 0.012 | − 6.72 ± 0.24 | 7.43 |
F6 | 1 | 35.0 | − 1 | 20.0 | 1 | 240.0 | 248.1 ± 1.9 | 0.053 ± 0.037 | − 6.48 ± 0.15 | 14.69 |
F7 | − 1 | 25.0 | 1 | 40.0 | 1 | 240.0 | 258.7 ± 4.5 | 0.198 ± 0.011 | − 6.35 ± 0.33 | 22.63 |
F8 | 1 | 35.0 | 1 | 40.0 | 1 | 240.0 | 206.4 ± 1.2 | 0.061 ± 0.045 | − 6.67 ± 0.30 | 2.88 |
Axial points | ||||||||||
F9 | 1.68 | 38.4 | 0 | 30.0 | 0 | 180.0 | 222.4 ± 2.4 | 0.033 ± 0.011 | − 5.63 ± 0.37 | 39.12 |
F10 | − 1.68 | 21.6 | 0 | 30.0 | 0 | 180.0 | 162.6 ± 0.4 | 0.262 ± 0.012 | − 6.83 ± 0.37 | 39.36 |
F11 | 0 | 30.0 | 1.68 | 47.0 | 0 | 180.0 | 226.7 ± 4.4 | 0.236 ± 0.011 | − 6.49 ± 0.25 | 19.94 |
F12 | 0 | 30.0 | − 1.68 | 13.0 | 0 | 180.0 | 196.8 ± 2.5 | 0.103 ± 0.056 | − 6.47 ± 0.55 | 43.10 |
F13 | 0 | 30.0 | 0 | 30.0 | 1.68 | 281.0 | 239.8 ± 0.7 | 0.056 ± 0.020 | − 5.77 ± 0.47 | 23.39 |
F14 | 0 | 30.0 | 0 | 30.0 | − 1.68 | 79.0 | 382.6 ± 5.2 | 0.221 ± 0.011 | − 5.93 ± 0.21 | 33.95 |
Center points | ||||||||||
F15 | 0 | 30.0 | 0 | 30.0 | 0 | 180.0 | 220.1 ± 5.6 | 0.059 ± 0.019 | − 5.36 ± 0.03 | 24.01 |
F16 | 0 | 30.0 | 0 | 30.0 | 0 | 180.0 | 222.1 ± 3.6 | 0.062 ± 0.021 | − 5.36 ± 0.11 | 23.23 |