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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Food-grade TiO2 is trapped by intestinal mucus in vitro but does not impair mucin O-glycosylation and short-chain fatty acid synthesis in vivo: implications for gut barrier protection

Fig. 4

MUC5AC immunofluorescence staining of the HT29-MTX cell line. Representative confocal laser scanning microscopy z-stack image of MUC5AC (in red) produced by HT29-MTX cells (E-cadherin, in green) cultured under standard conditions. A z-stack section (x, y) at the level of the mucus; B z-stack section (x, y) at the level of HT29-MTX cells with orthogonal x (y, z) and y (x, z) sections on the right and bottom, respectively. The image was prepared using the easy 3D section view of IMARIS software with extended view (signal gathered from a thickness of 5 µm)

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