Fig. 6

Quantification of cellular NP association for fluoresceinamine (FA) labeled blank (a1, a2) and mRNA complexed nanoparticles (b1, b2) tested in DC2.4 cells. NPs were incubated for 2 h and 4 h at different concentrations (20, 40 and 60 µg/mL corresponding to the weight ratios 1:10, 1:20 and 1:30 used for transfection). a1, b1 Green NP fluorescent signal quantified by flow cytometry. Blank LPNs tend to show more uptake then blank CS-PLGA NPs, whereas for mRNA-loaded nanoparticles the opposite was observed. None of the samples showed a significant difference between 2 and 4 h incubation. a2, b2 Representative graphs obtained for NP samples after 4 h incubation. N = 4, mean ± SD