Fig. 4

In vitro evaluation of detecting the targeting of HA-eNPs to CD44. a, b Coumarin-6 loaded HA-eNPs were incubated with CD44-enriched B16F10 cells or CD44-low expressing MCF-7 cells, respectively. Fluorescence signals were observed by confocal microscopy. c Flow cytometry analysis showing the cellular uptake percentage of the B16F10 and MCF-7 cells after incubation with free coumarin-6, coumarin-6 loaded NPs, or HA-eNPs. Data are shown as the means ± SD, ^**P < 0.01, ns not significant (n = 3). d, e B16F10 cells were preincubated with anti-CD44 antibody and followed by incubating with HA-eNPs for 4 h. Fluorescence signals were observed by confocal microscopy and flow cytometry. Data are shown as the means ± SD, ^**P < 0.01 (n = 3)