Fig. 4

Representative SEM images illustrating the impact of different surface coatings of ITO/glass substrates on ADs of particles. All experiments were performed with 200 nm silica particles with an input concentration of 50 µg/mL and 1 h incubation time for a, b without cells and c with cells. a Precoating with CCM, b precoating with conditioned CCM derived from A549 cell cultures and c intercellular region of an ITO/glass substrate overgrown with A549 cells. Small dark rectangles are electron beam induced perturbations. CCM cell culture medium. Scale bar: 5μm