Fig. 1

Oligomerization through self-assembly of hIGFBP-2_249–289 (R281C). a SDS-PAGE profile of the oligomerization M: marker, A: Monomer, B: Dimer (day 1), C: more populated dimer (day 2), D: multiple oligomer species (day 4); b 2D [¹⁵N, ¹H] heteronuclear single quantum correlation (HSQC) spectra on day 1 and day 4, purple color dotted line circles depict the pairs of peaks representing old and new species for some of the amino acid residues; c Cys44 was monitored for oxidation of –SH to disulfide (–SS–) with oligomerization, based on ¹³C^β shifts observed in the 2D [¹³C–¹H] HSQC NMR experiment, d TEM images of the hIGFBP-2_249–289 nanotubes