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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Direct isolation and characterization of circulating exosomes from biological samples using magnetic nanowires

Fig. 3

a–c Fluorescence images of exosomes captured on Abs_MNWs from the plasma of lung cancer patients. d–f Fluorescence images of exosomes that were immediately released from the Abs_MNWs after incubation with DTT for 30 min. Exosomes captured and released by the magnetic nanowires were detected under the fluorescence microscope following staining with DiO dye. All fluorescence images were acquired under same condition (scale bar, 10 µm; inset scale bar, 5 µm). g TEM images showing exosomes captured on Abs_MNWs from the plasma of lung cancer patients (scale bar, 500 nm; inset scale bar, 100 nm). Inset exhibits an image at higher magnification. h Representative TEM images showing exosomes released from the plasma of lung cancer patients using Abs_MNWs (scale bar, 50 nm). i Bioanalyzer size distribution of exosomal RNA that were extracted from exosomes isolated by Abs_MNWs

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