Fig. 3

MNP subcellular localisation in Jurkat and murine primary T cells. a Representative images of Jurkat and murine T cells after MNP treatment acquired by confocal microscopy [cell membrane (red), MNPs (gray) and nuclei (blue)] (scale bar = 10 μm). ImageJ software was used for orthogonal projections. b Perls’ Prussian blue staining and neutral red counterstaining of Jurkat and murine T cells after MNP treatment (scale bar = 10 μm). c Representative TEM images of Jurkat and murine T cells after MNP treatment. Whole cells (top panel pictures) were imaged as well as more detailed cell parts (bottom panel pictures) to better illustrate interactions between MNPs and cell surface. Arrowheads indicate MNP aggregates attached to the cell surface