Fig. 1
Influence of different Smix ratios and the P22 concentration on characterizations. a–c Influence of different Smix ratios on size, zeta potential and PDI of nanoemulsions: a particle sizes. b Zeta potentials. c Polydispersity indexes. d–g Influence of the P22 concentration on size, zeta potential, PDI and encapsulation efficacy: d particle sizes. e Zeta potentials. f Polydispersity indexes. g Encapsulation efficiencies. For d–f the results obtained were compared with blank nanoemulsion (epitope peptide concentration = 0). For g, the results obtained were compared with 250 μg/mL group. The data are expressed as the mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001