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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway

Fig. 1

In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro transfection efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells (a). β-actin mRNA was measured by QRT-PCR and then % remaining β-actin mRNA expression was calculated based on control as 100% (b). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl (c). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h (d). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, *p < 0.05, **p < 0.01 and ***p < 0.001

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