Fig. 3

The intracellular trafficking of CsA@PLGA-PEG-SS31 in H/R injured H9c2 cells. a The mitochondrial distribution and the fluorescence intensity after free coumarin 6, coumarin 6 labeled CsA@PLGA-PEG (coumarin 6@PLGA-PEG), and coumarin 6 labeled CsA@PLGA-PEG-SS31 (coumarin 6@PLGA-PEG-SS31) were incubated with H/R injured H9c2 cells for 4 h (60 × 10). The fluorescence intensity was graphed according to the white line of merged fluorescence images. b Colocalization efficiency of coumarin 6 after free coumarin 6, coumarin 6 labeled CsA@PLGA-PEG (coumarin 6@PLGA-PEG) and coumarin 6 labeled CsA@PLGA-PEG-SS31 (coumarin 6@PLGA-PEG-SS31) incubated with H/R injured H9c2 cells for 4 h (×60). **p < 0.01 versus coumarin 6 group, ^##p < 0.01 versus coumarin 6@PLGA-PEG group