Fig. 8

a CFU results of S. mutans treated and not with 5% toothpastes in saline solutions at various times. Bacterial survival is expressed in CFU rate compared to untreated one. Statistical analysis was performed by two-way analysis of variance (ANOVA) method coupled with the Bonferroni post-test. Different letters indicate different statistical significances. b S. mutans cell survival after treatment for 4 h in saline solutions with 1% of toothpastes content. Error bar indicates standard deviation. Statistical analysis was performed by one-way ANOVA method coupled with the Bonferroni post-test (**P < 0.01 compared to the control). c Biofilm biomass analysis on CV stained deciduous teeth. Data are expressed as percentage of biofilm formation relative to control teeth brushed with Neutro-pasta toothpaste. Histograms are the mean of three independent experiments. Error bars indicate SD and statistical analysis was performed by one-way ANOVA method coupled with the Bonferroni post-test (ns not significant). d Photographs of teeth brushed with the different types of toothpaste and stained with CV after S. mutans biofilm growing