Fig. 2

Characterization of unmodified exosomes and the RGD-exo loaded with miR-210. a Western blot analysis of Alix, TSG101, and calnexin expression in cells and in exosomes isolated from their conditioned medium. The supernatant obtained from ultracentrifugation during exosome isolation was used as the negative control. b Schematic diagram of functionalized exosomes with DBCO groups and c(RGDyK) peptides. c Transmission electron micrograph of the exosomes, RGD-exo, and RGD-exo loaded with miR-210 (triangles). Scale bar: 500 nm. d Size distribution of unmodified exosomes, the RGD-exo, and the RGD-exo:miR-210 detected by NTA measurements