Fig. 7

Analysis of the neuronal marker microtubule-associated protein 2 (MAP-2) was performed at day in vitro (DIV) 7 to evaluate the effect of SiPCL-NP-exposure for 24 h at DIV1 (a) and DIV4 (b) and DIV6 (c) on the state of differentiation in SH-SY5Y cells. Undifferentiated cells (Undiff) and cells differentiated with retinoic acid (RA) or staurosporine (ST) were examined in parallel. The histogram depicts MAP-2 levels in relation to actin (loading control). A representative Western blot for SiPCL-NP-exposure on DIV4 is shown above the respective histogram. Image J was used for the analysis. Error bars correspond to SEM. Significant differences between control- and NP-exposed-groups are labeled with asterisks (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001)