Fig. 3
Colony formation of NCH-421K cells after different treatments. a Clone numbers after different treatments on day 7 (n = 10; *P < 0.05, **P < 0.01, ***P < 0.001). b Tumorsphere changes in 1 week after different treatments (n= 4; ***P < 0.001). c Images of tumorspheres after different treatments. NCH-421K cells at a density of 2.5 × 105 cells/well were plated into 12-well plates and cultured overnight. Cells were treated with 100 μM TMZ or TMZ/PSi NPs for 24 h. Bar: 100 μm