Fig. 7From: Using 3D gastrointestinal tract in vitro models with microfold cells and mucus secreting ability to assess the hazard of copper oxide nanomaterialsCuO NM and CuSO4 cellular uptake and translocation across the co-culture barrier. The Caco-2/Raji B and Caco-2/HT29-MTX co-cultures were exposed to CuO NMs or CuSO4 at concentrations of 3.17, 6.34 or 12.68 Cu µg/cm2 for 24 and 48 h. The level of Cu in the AP (a) and BL (b) compartments, and the cells (c) was evaluated by ICP-OES. Data are expressed as mean copper concentration (as a percentage of the exposed concentration) ± SEM (n = 3). Significance at p < 0.05 is indicted by * for comparison of CuO NMs at a concentration of 3.17 Cu µg/cm2 to the other treatment concentrations within each time point or # for comparison of equivalent concentrations between 24 and 48 h time pointsBack to article page