Fig. 4

Functional assessment of OT-I CD8⁺ T cells after association with APS-MNPs. a Conjugation kinetics of OT-I CD8⁺ T cells after treatment with APS-MNPs, with the EG7-OVA cell line, co-incubated at a 1: 1 ratio. b Degranulation capacity of OT-I CD8⁺ T cells after being treated with different concentrations of APS-MNPs and co-incubated with the EG7-OVA cell line. c Cytolytic activity of OT-I CD8⁺ T cells after being treated with different concentrations of APS-MNPs, against EG7-OVA cell line, co-incubated at different ratios for 5 h. d Quantification of the percentage of OT-I CD8⁺ T cell IFN-γ⁺ after exposure to a variety of stimuli. Mean values ± SD are shown for three or four different experiments, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001