Fig. 7

Blood circulation and biodistribution of different drug formulations. a Plasma DOX or DOX prodrug concentration as a function time after intravenous injection of free DOX and ATD-NPs. Data showed mean ± SD, n = 3. b, d Distribution of total DOX in organs or tissues at 4 h (b), 12 h (c), and 24 h. Data presented mean ± SD, n = 3. d After a single intravenous administration of free DOX, TD-NPs, and ATD-NPs, respectively. e In vivo fluorescence imaging of the PC-3 tumor bearing mice at 12 h, 24 h, 36 h, and 48 h after a single intravenous administration of cy5.5 loaded ATD-NPs and TD-NPs. f Ex vivo fluorescence images of isolated tissues at 48 h post-injection