Fig. 2From: A novel biotinylated nanobody-based blocking ELISA for the rapid and sensitive clinical detection of porcine epidemic diarrhea virusIdentification of PEDV N protein specific nanobodies. A periplasmic extract ELISA detected 98 clones, of which 19 clones were identified as positive. The absorbance of the reaction mixture in an antigen-coated well was at least threefold greater than that of the reaction mixture in a PBS-coated well and considered positiveBack to article page